Takara Protein digestion for structural analysis: Pfu Protease S
단백질 및 펩타이드 1차 구조 분석용 고온 안정성 프로테아제. 85–95°C에서 최적 활성, SDS·요소·아세토니트릴 등 변성 조건에서도 높은 내성. Recombinant Bacillus에서 생산된 Pyrococcus furiosus 유래 효소. 연구용 단백질 소화에 적합.
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Takara Protein Digestion for Structural Analysis: Pfu Protease S
Applications
- Digestion of proteins and peptides for primary structure analysis
Source
Recombinant Bacillus encoding the Pyrococcus furiosus protease gene
Properties
| 항목 | 내용 |
|---|---|
| Molecular weight | 45 kDa (SDS-PAGE), 42.906 kDa (calculated) |
| Optimum temperature | 85–95°C |
| Optimum pH | 6.0–8.0 |
| Inhibitor | PMSF |
| Tolerance to denaturants (95°C, pH 7.0) | - 1% SDS: retains 50% activity after 24 hr - 6.4 M urea: retains 70% activity after 1 hr - 50% acetonitrile: retains 90% activity after 1 hr |
Definition of Activity
One unit of activity corresponds to the amount of enzyme required to hydrolyze 1 mmol of N-succinyl Ala-Ala-Pro-Phe p-nitroanilide in 1 minute at 95°C and pH 7.0.
Form
In a solution of 25 mM Tris-HCl (pH 7.6) containing 40% ethanol
Additional Product Information
Please see the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications. Refer to the Kit Components List under the Documents tab for component information.
Takara Bio USA, Inc.
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Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Additional product and intellectual property information available at takarabio.com.
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