Takara Protein digestion for structural analysis: Pfu Protease S

More Information

Applications

• Digestion of proteins and peptides for primary structure analysis

Source

Recombinant Bacillus encoding the Pyrococcus furiosus protease gene

Properties

Molecular weight: 45 kDa (SDS-PAGE), 42.906 kDA (calculated)
Optimum temperature: 85-95°C
Optimum pH: 6.0-8.0
Inhibitor: PMSF
Tolerance to denaturants at 95°C pH 7.0:

• 1% SDS; retains 50% activity after 24 hr at 95°C, pH 7.0
• 6.4 M urea; retains 70% activity after 1 hr at 95°C, pH 7.0
• 50% acetonitrile; retains 90% activity after 1 hr at 95°C, pH 7.0

Definition of activity

One unit of activity corresponds to the amount of enzyme required to hydrolyze 1 mmol of N-succinyl Ala-Ala-Pro-Phe p-nitroanilide in 1 minute at 95°C and pH 7.0.

Form

In a solution of 25 mM Tris-HCl (pH 7.6) containing 40% ethanol

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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