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ELK Biotechnology DDDDK-Tag(binds to flag sequnence) rabbit pAb
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ELK Biotechnology DDDDK-Tag(binds to flag sequnence) rabbit pAb

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FLAG 태그 서열(DYKDDDDK)을 인식하는 rabbit polyclonal antibody로 다양한 단백질 태그 검출에 사용. WB, ELISA, IP, IF 등 다중 응용 가능. 고순도 IgG 형태로 제공되며 -20°C에서 1년간 안정 저장. 단백질 발현 및 정제 연구에 적합.

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pk
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ELK Biotechnology ES1078-100UL DDDDK-Tag(binds to flag sequnence) rabbit pAb, 100UL pk판매 단위 pk ·
재고 확인 필요
402,000원VAT 포함 442,200원
ELK Biotechnology ES1078-50UL DDDDK-Tag(binds to flag sequnence) rabbit pAb, 50UL pk판매 단위 pk ·
재고 확인 필요
301,000원VAT 포함 331,100원

ELK Biotechnology · ELK Biotechnology DDDDK-Tag(binds to flag sequnence) rabbit pAb

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DDDDK-Tag (binds to FLAG sequence) Rabbit pAb

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항목 내용
Product Name DDDDK-Tag (binds to FLAG sequence) Rabbit pAb
Alternative Names DDDDK epitope tag; DYKDDDDK epitope tag
Applications WB, ELISA, IP, IF
Recommended Dilutions Western Blot: 1/1000–1/3000
ELISA: 1/5000–1/20000
Not yet tested in other applications
Immunogen DDDDK synthetic peptide conjugated to KLH
Species Reactivity Species independent
Clonality Polyclonal
Isotype IgG
Concentration 1 mg/ml
Storage -20°C / 1 year

Background

The DYKDDDDK (FLAG) peptide has been widely used as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is hydrophilic, allowing it to be located on the surface of fusion proteins and easily accessible to antibodies.
A FLAG-tag can be utilized in various assays requiring antibody recognition, including:

  • Western blotting
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow cytometry
  • Protein purification
  • Protein-protein interaction studies
  • Cell ultrastructure and protein localization research

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