
ELK Biotechnology Flag-Tag Rabbit pAb
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| Product name: | Flag-Tag Rabbit pAb |
| Reactivity: | N/A |
| Alternative Names: | N/A |
| Source: | Rabbit |
| Applications: | WB |
| Species: | N/A |
| Recommended dilutions: | WB 1:5,000 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1mg/ml |
| Observed band: | N/A |
| GeneID(Human): | N/A |
| Human Swiss-Prot No: | N/A |
| Cellular localization: | N/A |
| Background: | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
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