Thermo Fisher Scientific eIF3F Polyclonal Antibody
eIF3F 단백질을 표적으로 하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 4°C에서 보관하며, 액상 형태로 제공됩니다. 단백질 번역 개시 복합체 연구용으로 권장됩니다.
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- A303005Ax (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific eIF3F Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 307 and 357 of human Eukaryotic Translation Initiation Factor 3, Subunit F |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee.
Target Information
The eukaryotic translation initiation factor 3 (eIF-3) complex is essential for multiple steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC).
The eIF-3 complex promotes mRNA recruitment to the 43S PIC and scanning for AUG recognition, and is also involved in disassembly and recycling of post-termination ribosomal complexes, preventing premature joining of 40S and 60S subunits prior to initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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