Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), Pacific Orange

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BM8

Immunogen

mouse F4/80 which is a macrophage-restricted cell surface glycoprotein.

Conjugate

Pacific Orange™ Pacific Orange™ Pacific Orange™

View additional formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 647
• APC
• Pacific Blue
• PE
• PE-Texas Red
• Request custom conjugation

Excitation/Emission Max

400/551 nm View spectra

Form

Liquid

Concentration

0.3 mg/mL

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539704

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.