Thermo Fisher Scientific NPAT Polyclonal Antibody
Thermo Fisher Scientific의 NPAT Polyclonal Antibody는 인간 NPAT 단백질을 인식하는 토끼 IgG 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 4°C에서 보관합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1377 and 1427 of human Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT, UniProt ID: Q14207-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Predicted to react with Mouse based on 100% sequence identity
Target Information
NPAT is required for progression through the G1 and S phases of the cell cycle and for S phase entry. It activates transcription of histone H2A, H2B, H3, and H4 genes in conjunction with MIZF, and positively regulates ATM, MIZF, and PRKDC promoters. Transcriptional activation may involve recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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