Thermo Fisher Scientific CD163 Monoclonal Antibody (M130, 2163)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and a member of the scavenger receptor cysteine-rich superfamily. It acts as a receptor for the hemoglobin-haptoglobin complex, protecting tissues from oxidative damage and participating in iron recycling via endocytosis and heme breakdown.

CD163 is expressed exclusively on human monocytes and macrophages, predominantly in the late phase of inflammation. It is found on all circulating monocytes and most tissue macrophages, except those in mantle zones, germinal centers, interdigitating reticulum cells, and Langerhans cells. Expression is high on CD14⁺ and CD16⁺ monocytes and upregulated by IL-10, IL-6, and dexamethasone. LPS and PMA induce shedding of CD163 from the cell surface.

CD163 binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, showing higher affinity for multimeric haptoglobin (HP1F phenotype). It also triggers intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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