Thermo Scientific™

HotStart-IT Binding Protein

HotStart-IT Binding Protein is the active component in a novel hot start technology called primer sequestration.

HotStart-IT Binding Protein is the active component in the novel hot start technology known as primer sequestration. In primer sequestration, hot start PCR methods reduce or eliminate non-specific primer-extension products formed at lower temperatures during assembly of PCR reactions. At these less stringent annealing temperatures, primers may bind non-specifically, which often leads to unwanted amplification products and primer-dimers. To resolve this problem, we have produced a high-quality DNA binding protein that is especially useful at sequestering primers at lower temperatures, making them unavailable for use by a polymerase (see figure).

사양

용도(애플리케이션)Hot-start PCR

배송 조건Dry Ice

함께 사용가능한 버퍼1X PCR Reaction Buffer

수량400 μg (200 x 25 μL reactions)

제품 유형HotStart-IT Binding Protein

Unit SizeEach