Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), PE-Cyanine7, eBioscience
Mouse PD-1 단백질을 인식하는 J43 클론 항체로, PE-Cyanine7 형광체로 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 488~561 nm 레이저로 여기됩니다. 면역조절 연구 및 T세포 활성 분석에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) | - | 1 publication |
| Flow Cytometry | 1 µg/test | 24 publications |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J43 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10853672 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 480 / 605 / 650 / 786
- eFluor 450 / 506
- FITC
- Functional Grade
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available
Product Specific Information
The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is mainly expressed on activated T and B lymphocytes. Its ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), show overlapping functions and are expressed on normal tissues and antigen-presenting cells.
J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo, J43 enhances contact hypersensitivity and exacerbates acute GVHD, EAE, and NOD diabetes, similar to PD-1 deficiency or PD-L1/PD-L2 blockade.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes at ≤1 µg/test. Optimal titration recommended.
Light Sensitivity: Protect from light due to photo-induced oxidation of tandem dye.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54).
Excitation / Emission: 488–561 nm / 775 nm; compatible with Blue, Green, Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
PD-1 (programmed death-1, PDCD-1) is expressed on activated T cells and delivers inhibitory signals upon binding to PD-L1 or PD-L2. This interaction limits T-cell activation in the absence of danger signals. PD-1 deficiency leads to hyperactive immune responses and autoimmune diseases. Although predicted at 55 kDa, PD-1 often migrates at a higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures.
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