Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), PE-Cyanine7, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 480 / 605 / 650 / 786
• eFluor 450 / 506
• FITC
• Functional Grade
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom conjugation available

Product Specific Information

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is mainly expressed on activated T and B lymphocytes. Its ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), show overlapping functions and are expressed on normal tissues and antigen-presenting cells.

J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo, J43 enhances contact hypersensitivity and exacerbates acute GVHD, EAE, and NOD diabetes, similar to PD-1 deficiency or PD-L1/PD-L2 blockade.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes at ≤1 µg/test. Optimal titration recommended.
Light Sensitivity: Protect from light due to photo-induced oxidation of tandem dye.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54).
Excitation / Emission: 488–561 nm / 775 nm; compatible with Blue, Green, Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

PD-1 (programmed death-1, PDCD-1) is expressed on activated T cells and delivers inhibitory signals upon binding to PD-L1 or PD-L2. This interaction limits T-cell activation in the absence of danger signals. PD-1 deficiency leads to hyperactive immune responses and autoimmune diseases. Although predicted at 55 kDa, PD-1 often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures.

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