Thermo Fisher Scientific Precision ID mtDNA Whole Genome Panel

Applied Biosystems™ Precision ID mtDNA Whole Genome Panel

The Applied Biosystems Precision ID mtDNA Whole Genome Panel is an innovative next-generation sequencing (NGS) approach to mitochondrial DNA (mtDNA) analysis specifically developed for forensic applications.
It is a 2-pool multiplex assay that targets the entire human mitochondrial genome (16,569 bp). Each pool contains 81 primer pairs with minimal primer overlap between pools.
This panel uses a tiling approach to obtain optimal coverage data from highly compromised, degraded samples such as hair shafts, teeth, and bones, built on Ion AmpliSeq™ technology.

This mtDNA tiling approach was also used to construct the Precision ID mtDNA Control Region Panel (Cat. No. A31443), which targets only the control region of the genome.

주요 특징

Simplicity

• Small amplicons enable better recovery of mtDNA genome with degraded samples
• As little as 2 pg of input
• Automated library and template preparation using the Ion Chef™ System
• Optimized analysis using Converge™ Software

Scalability

• Multiple chip formats to meet a range of sample throughput requirements
• Ability to multiplex up to 32 samples*

Speed

• Less than 45 minutes of hands-on time for a DNA-to-data targeted sequencing workflow*
• Total sample-to-data time of less than 2 days*

*Using the Precision ID NGS System for Human Identification

제품 개요

Mitochondrial DNA analysis is a key DNA detection method used by forensic scientists when samples such as teeth, bones, and hairs without follicles are collected from crime scenes, missing person remains, or disaster areas where nuclear DNA is insufficient in quality or quantity.

Balanced coverage is achieved using a small amplicon design (163 bp average) and degenerate primers that align with primer-binding SNPs, increasing success with degraded and low-template DNA samples.
This panel can be used with all types of forensic samples, including ancient DNA remains and clinical research specimens.

Full automation of library and template preparation using the Ion Chef System and sequencing on Ion GeneStudio™ series systems reduces variation and minimizes hands-on time.
The Converge NGS Analysis module automates mtDNA analysis, leveraging optimized base calling, phylogenetically guided alignment, and quality filtering algorithms specific to the Precision ID mtDNA panels.
Variant calling, including heteroplasmy detection, can be easily optimized within the software.

These solutions provide forensic DNA laboratories flexibility to detect variation within noncoding control sequences using the Precision ID mtDNA Control Region Panel, or to utilize full mtGenome sequence data using the Precision ID mtDNA Whole Genome Panel.

The panel provides sufficient material for:

• 96 reactions using the manual Precision ID Library Kit (Cat. No. A26435)
• 48 reactions using the automated Precision ID DL8 Library Kit (Cat. No. A33212)

Note: Precision ID mtDNA Whole Genome Panel, using control region data for analysis, is approved for inclusion in the US National DNA Index System (NDIS) CODIS database.

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