Thermo Fisher Scientific CTNNA3 Recombinant Rabbit Monoclonal Antibody (ARC2463)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC2463

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 700-895 of human CTNNA3 (Q9UI47) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CTNNA3,uniProtId:Q9UI47-1,ncbiNodeId:9606,antigenRange:700-895,antigenLength:895,antigenImageFileName:MA5-37908_CTNNA3_Q9UI47-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37908_CTNNA3_Q9UI47-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897828

Product Specific Information

Positive Samples: PC-3, Mouse testis, Mouse brain, Mouse heart, Rat testis, Rat brain

Immunogen sequence: DIIVLAKNMC MIMMEMTDFT RGKGPLKHTT DVIYAAKMIS ESGSRMDVLA RQIANQCPDP SCKQDLLAYL EQIKFYSHQL KICSQVKAEI QNLGGELIMS ALDSVTSLIQ AAKNLMNAVV QTVKMSYIAS TKIIRIQSPA GPRHPVVMWR MKAPAKKPLI KREKPEETCA AVRRGSAKKK IHPLQVMSEF RGRQIY

Target Information

Catenins are proteins that connect cadherin/beta-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alphaT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human alphaTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that alphaT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking alpha-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other alpha-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with alphaT-catenin cDNA, interaction with alpha-catenin was demonstrated by coimmunoprecipitation. The 892_24D2S antibody against alphaT-catenin does not show any cross reaction with alphaE-catenin or alphaNcatenin in western blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.