Thermo Fisher Scientific CTNNA3 Recombinant Rabbit Monoclonal Antibody (ARC2463)
CTNNA3 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 발현 시스템에서 생산된 재조합 항체로 고순도 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC2463 |
| Immunogen | Recombinant fusion protein corresponding to amino acids 700–895 of human CTNNA3 (Q9UI47) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897828 |
Product Specific Information
- Positive Samples: PC-3, Mouse testis, Mouse brain, Mouse heart, Rat testis, Rat brain
- Immunogen Sequence:
DIIVLAKNMC MIMMEMTDFT RGKGPLKHTT DVIYAAKMIS ESGSRMDVLA RQIANQCPDP SCKQDLLAYL EQIKFYSHQL KICSQVKAEI QNLGGELIMS ALDSVTSLIQ AAKNLMNAVV QTVKMSYIAS TKIIRIQSPA GPRHPVVMWR MKAPAKKPLI KREKPEETCA AVRRGSAKKK IHPLQVMSEF RGRQIY
Target Information
Catenins are proteins that connect cadherin/beta-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alphaT-catenin is expressed in other tissues, with the highest levels in the heart. Immunohistochemical analysis shows human alphaT-catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It can restore cell-cell adhesion in colon cancer cells lacking alpha-catenins, indicating its role in forming specific cell-cell contacts in muscle cells. The encoded protein has a predicted size of ~100 kDa, sharing 57% amino acid identity with other alpha-catenins. In cells transfected with alphaT-catenin cDNA, interaction with alpha-catenin was confirmed by coimmunoprecipitation. The 892_24D2S antibody against alphaT-catenin shows no cross-reactivity with alphaE-catenin or alphaN-catenin in western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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