Thermo Scientific™

Phusion Site-Directed Mutagenesis Kit

Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in자세히 알아보기

Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competent E. coli cells.

The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.

**
Highlights**

• Robust and reliable exponential amplification method
• No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid
• No need to destroy the starting template in a separate step
• Phusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutations
• Amplification of large plasmids up to 10 kb
• Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR
• T4 DNA Ligase included in the kit; no purification steps before or after ligation
• Compatible with all strains of competent E. coli cells

사양

형태Solution

형식Kit

GC-Rich PCR PerformanceHigh

중합효소Phusion Hot Start II High Fidelity DNA Polymerase

반응 속도Fast

돌연변이 유도 기능Single-Site Mutagenesis

제품라인Phusion

제품 유형Site-Directed Mutagenesis Kit

수량20 reactions

Fidelity (Taq 대비)52X

핫 스타트Yes

반응 수20 Reactions

시작 물질DNA

Unit SizeEach