Thermo Fisher Scientific DENND1B Polyclonal Antibody
Rabbit polyclonal antibody against human DENND1B. Validated for WB, IHC(P), and Flow Cytometry. Recognizes N-terminal region (aa 39–68). Suitable for research on clathrin-mediated endocytosis and RAB35 signaling.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 39–68 of the N-terminal region of human DENND1B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2541385 |
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
Clathrin-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 and bind to clathrin and clathrin adaptor protein-2 (AP2). Thus, connecdenns link RAB35 activation with the clathrin machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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