Thermo Fisher Scientific IgD Monoclonal Antibody (11-26c (11-26)), FITC, eBioscience
마우스 IgD 델타 중쇄를 인식하는 단클론 항체로, FITC 형광 표지되어 있습니다. Flow cytometry 분석에 최적화되어 있으며, 0.125 µg/test로 사용 가능합니다. 비활성화형 항체로 B 세포 활성화 없이 표면 IgD 검출에 적합합니다.
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Thermo Fisher Scientific IgD Monoclonal Antibody (11-26c (11-26)), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 28 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11-26c (11-26) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465346 |
Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG, or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.
Applications Reported:
Used for flow cytometric analysis to detect surface IgD on mouse B cells.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.125 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.
Excitation / Emission / Laser:
488 nm / 520 nm / Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IgD is a type I transmembrane protein co-expressed with membrane IgM on mature naive B cells. It also exists as a soluble form in serum and participates in signal transduction via the B cell receptor upon antigen binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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