Thermo Fisher Scientific Lewis A (Blood Group Antigen) Monoclonal Antibody (SPM522)
Lewis A 혈액형 항원을 인식하는 단일클론 항체로, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. 인간 및 마우스 반응성. 단백질 A/G 정제, PBS 용액 형태. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM522 |
| Immunogen | Mucins isolated from ovarian cyst fluid |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids synthesized by exocrine epithelial cells and circulating in body fluids. These glycosphingolipids are involved in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells, determining their Lewis phenotype. The gene encoding this antigen belongs to the fucosyltransferase family, catalyzing the addition of fucose to precursor polysaccharides in the final step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)- and alpha(1,4)-fucosyltransferase activities. Mutations in this gene cause Lewis antigen-negative phenotypes. Multiple alternatively spliced variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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