Thermo Fisher Scientific GOLPH3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.3 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide with sequence C-DLATKRVRQLLDLD (aa 262-275). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GOLPH3,uniProtId:Q9H4A6-1,ncbiNodeId:9606,antigenRange:262-275,antigenLength:298,antigenImageFileName:PA5-142534_GOLPH3_Q9H4A6-1_Goat.svg,antigenImageFileNamePDP:PA5-142534_GOLPH3_Q9H4A6-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2933181

Product Specific Information

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.

Target Information

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signaling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.