Thermo Fisher Scientific ALAD Polyclonal Antibody
인간 ALAD 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 IHC(P) 실험에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ALAD. Recombinant protein control fragment (Product # RP-93005) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2637828 |
Product Specific Information
Immunogen sequence:
GCQVVAPSDM MDGRVEAIKE ALMAHGLGNR VSVMSYSAKF ASCFYGPFRD AAKSSPAFGD RRCYQLPPGA RGLALRAVDR D
Sequence identity:
Highest antigen sequence identity to orthologs: Mouse – 94%, Rat – 94%.
Target Information
The ALAD enzyme is composed of eight identical subunits and catalyzes the condensation of two molecules of delta-aminolevulinate to form porphobilinogen, a precursor of heme, cytochromes, and other hemoproteins.
ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, and zinc is essential for its enzymatic activity.
ALAD activity is inhibited by lead, and defects in the ALAD structural gene can increase sensitivity to lead poisoning and acute hepatic porphyria.
Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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