Thermo Fisher Scientific Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Monoclonal Antibody (A53-B/A2.26)
인간 세포에서 Cytokeratin 19(KRT19)를 검출하는 마우스 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. 단백질 A/G로 정제된 액상 항체로, 보존제 없이 PBS(pH 7.4)에 저장. -20°C에서 보관, 동결-해동 반복은 피해야 함.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A53-B/A2.26 |
| Immunogen | Human breast cancer MCF-7 cells (target: Cytokeratin 19, UniProt ID: P08727-1, antigen range: 1–400) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Rat or Ferret.
Target Information
Cytokeratin 19 is part of a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40–68 kDa and isoelectric pH between 4.9–7.8.
Expression patterns of cytokeratin subtypes are characteristic of epithelial type and degree of differentiation, aiding in distinguishing epithelial malignancies.
Cytokeratin antibodies assist in tumor differential diagnosis using immunohistochemistry, cytopathology, and flow cytometry.
Cytokeratin 19 is not expressed in hepatocytes, making it useful for identifying liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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