
Thermo Fisher Scientific GFP Recombinant Rabbit Monoclonal Antibody (JF51-05), HRP
GFP 단백질을 인식하는 HRP-conjugated recombinant rabbit monoclonal antibody. Western blot에 적합하며 높은 특이성과 민감도를 제공. 단백질 A 정제, 액상 형태, 1 mg/mL 농도. 연구용으로 GFP 태그 단백질 검출에 최적화.
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Applications
- Western Blot (WB): 1:10,000–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF51-05 |
| Immunogen | Recombinant full length protein of Aequorea victoria GFP |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2931466 |
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular systems.
As a fusion tag, GFP enables localization studies, movement tracking, and analysis of subcellular compartment dynamics. GFP technology has provided significant insights into cellular physiology.
GFP is a 27 kDa monomeric protein that forms its fluorescent pigment autocatalytically. It absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without requiring additional cofactors. Its fluorescence is stable and species independent, making it suitable for various applications such as:
- Monitoring gene expression and protein localization
- Studying protein–protein interactions (e.g., yeast two-hybrid system)
- Measuring distances between proteins via FRET experiments
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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