Thermo Fisher Scientific CHFR Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (KFNHICEQTRFKN) corresponding to the C-terminus amino acids of CHFR (aa 652-664). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CHFR,uniProtId:Q96EP1-1,ncbiNodeId:9606,antigenRange:652-664,antigenLength:664,antigenImageFileName:PA5-18127_CHFR_Q96EP1-1_Goat.svg,antigenImageFileNamePDP:PA5-18127_CHFR_Q96EP1-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_11000351

Product Specific Information

This antibody is predicted to react with bovine, mouse, porcine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

Target Information

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the Lys-48-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of Lys-63-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at Lys-63 are usually not targeted for degradation, but are rather involved in signaling cellular stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.