Thermo Fisher Scientific SF1 Polyclonal Antibody
Thermo Fisher Scientific SF1 Polyclonal Antibody는 인간 SF1 단백질을 인식하는 Rabbit IgG 폴리클로날 항체로, WB, ICC/IF, IP에 사용 가능합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 다양한 종에서 높은 예측 반응성을 보입니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific SF1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–309 of Human splicing factor 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.77 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545530 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji
- Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (94%), Xenopus laevis (96%), Rabbit (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor that recognizes the intron branch point sequence at the 3′ splice site together with the large subunit of U2 auxiliary factor (U2AF). It is essential for early spliceosome assembly and plays roles in nuclear pre-mRNA retention and transcriptional repression.
The protein includes:
- N-terminal U2AF ligand motif
- Central hnRNP K homology motif and quaking 2 region
- Zinc knuckles domain
- C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-28054_SF1_Q15637-1_Rabbit.svg, PA5-28054_SF1_Q15637-1_Rabbit_PDP.jpeg)
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