Thermo Fisher Scientific CD8 Monoclonal Antibody (SK1)

Applications and Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 2–10 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 0.5–5 µg/10⁶ cells

Product Specifications

Product Specific Information

• Positive control: Spleen
• Cellular location: Cell membrane

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It plays an essential role in signal transduction, T cell differentiation, and activation.
CD8 is mainly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with the T cell receptor (TCR) for MHC class I molecules.
It is also found on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8α homodimers.
Binding of MHC-I/peptide complexes to CD8 triggers recruitment of Lck kinase, promoting lymphokine production, motility, and activation of cytotoxic T lymphocytes (CTLs).
Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.