Norgen Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

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Norgen Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

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소변 내 탈락 세포 또는 세균으로부터 고품질 DNA를 빠르고 효율적으로 정제하는 키트. 1~50mL 소변 샘플에 적용 가능하며, PCR·qPCR 등 민감한 분석에 적합. 그람양성·음성 세균 모두 분리 가능. 10분 내 10회 정제 가능.

브랜드: Norgen
카테고리: Genomic DNA Purification Systems and Kits
판매단위: pk

#Norgen #Reagents #R #DNA Reagents

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Norgen · Norgen Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

Norgen Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

SKU: 47050
Rapid and efficient purification of DNA from exfoliated cells or bacteria in urine.

Features and Benefits

Isolate genomic DNA from exfoliated cells or bacteria found in urine
Process as little as 1 mL and up to 50 mL of urine
Obtain high-quality DNA for sensitive downstream applications
Rapid processing time

Product Description

Norgen’s Urine DNA Isolation Kit for Exfoliated Cells or Bacteria enables rapid isolation of:

Human genomic DNA from exfoliated cells shed into urine from the urinary tract
Bacterial genomic DNA from urine samples

The kit isolates DNA from 1–50 mL urine. The purified DNA can be used for diagnostic and research applications, including detection and monitoring of bladder, kidney, or urinary-tract cancers. It also isolates bacterial DNA from both human and animal urine to study bacterial levels and types present. The kit effectively isolates DNA from both Gram-negative and Gram-positive bacteria, including E. coli, Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp., Clostridial spp., Leptospirosis spp., Chlamydia trachomatis, and Neisseria gonorrhoeae.

Typical yields vary depending on cell or bacterial density, which is influenced by health, diet, and sex. The kit is sensitive enough to isolate DNA from <10,000 CFU/mL bacteria found in healthy individuals. Purified DNA is fully digestible with all tested restriction enzymes and compatible with PCR, qPCR, and Southern blot analyses.

Kit Specifications

Specification	Description
Minimum Urine Input	1 mL
Maximum Urine Input	50 mL
Time to Complete 10 Purifications	10 minutes (+30 min incubation for bacteria, +15 min for exfoliated cells)

Storage Conditions and Stability

All solutions should be tightly sealed and stored at room temperature. The kit is stable for 2 years from shipment. Proteinase K is provided in a buffered storage solution and remains stable for up to 2 years at room temperature.

Kit Components

Component	Cat. 47050 (50 preps)
Resuspension Solution A	20 mL
Lysis Buffer B	40 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Proteinase K in Storage Buffer	1.2 mL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Supporting Data

Figure 1. Isolation and Detection of Human DNA from 1 mL Urine

Genomic DNA was isolated from exfoliated cells found in a 1 mL urine sample using Norgen’s kit.
PCR amplification using human beta-actin primers confirmed successful detection.

그래프 요약 (OCR 데이터):

항목	내용
X축	Cycle
Y축	PCR Base Line Subtracted CF RFU
세포 농도	150, 1,500, 15,000, 150,000 Cells/mL
범례	🔺 DNA Standards / 🔷 DNA from Urine / 🔶 No Template

이 그래프는 서로 다른 세포 농도에 따른 PCR 증폭 신호 변화를 보여줍니다.

Figure 2. Isolation and Detection of Bacterial DNA from 1 mL Urine

Bacterial genomic DNA was isolated from a 1 mL urine sample from a healthy individual.
PCR amplification confirmed detection even at low bacterial concentrations (<10,000 CFU/mL).

그래프 요약 (OCR 데이터):

항목	내용
X축	Cycle
Y축	PCR Base Line Subtracted CF RFU
세포 농도	1,500 / 15,000 / 1.5 million Cells/mL
범례	🔺 DNA Standard / 🔷 Urine from Healthy Individual / 🔶 No Template

이 그래프는 서로 다른 세포 농도에서의 PCR 증폭 결과를 비교한 것입니다.

Figure 3. Isolation of Genomic DNA from Gram-Positive and Gram-Negative Bacteria

Genomic DNA was isolated from urine samples spiked with B. cereus (Gram-positive) and E. coli (Gram-negative).
DNA was successfully recovered from both bacterial types.

샘플 구조 (OCR 데이터):

구분	샘플 번호	세균명
M	-	Marker
1	1	B. cereus
2	2	B. cereus
3	3	E. coli
4	4	E. coli

Documentation

FAQs

Q1. Why is my genomic DNA yield low?

Few cells or bacteria may be present in urine (<10,000 CFU/mL typical for healthy individuals).
Incomplete lysis: extend Proteinase K digestion or use fresh samples.
Incomplete elution: ensure centrifugation at 14,000 RPM after initial spin.

Q2. Why is my genomic DNA sheared?

Handle samples gently; minimize vortexing (≤15 sec).
Use fresh urine to prevent degradation by DNases and proteases.

Q3. Why does DNA perform poorly in PCR?

Inhibitors may be present in urine. Perform an additional wash with Resuspension Solution A + Proteinase K before lysis.

Q4. Can Proteinase K be purchased separately?

Yes, available as Product No. 28229 (5 × 1 mL tubes).

Citations

Title	Journal	Year	Authors
Genomic complexity of urothelial bladder cancer revealed in urinary cfDNA	European J of Human Genetics	2016	Fiona S Togneri et al.
Performance of the HSV OligoGen kit for diagnosis of herpes simplex virus type 1 and 2	Diagnostic Microbiology and Infectious Disease	2016	Parra-Sánchez M. et al.

제품 이미지

Figure 1. Isolation and Detection of Human DNA from 1 mL Urine
Figure 2. Isolation and Detection of Bacterial DNA from 1 mL Urine
Figure 3. Isolation of Gram-Positive and Gram-Negative Bacteria