Norgen Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

Norgen Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

SKU: 47050
Rapid and efficient purification of DNA from exfoliated cells or bacteria in urine.

Features and Benefits

• Isolate genomic DNA from exfoliated cells or bacteria found in urine
• Process as little as 1 mL and up to 50 mL of urine
• Obtain high-quality DNA for sensitive downstream applications
• Rapid processing time

Product Description

Norgen’s Urine DNA Isolation Kit for Exfoliated Cells or Bacteria enables rapid isolation of:

1. Human genomic DNA from exfoliated cells shed into urine from the urinary tract
2. Bacterial genomic DNA from urine samples

The kit isolates DNA from 1–50 mL urine. The purified DNA can be used for diagnostic and research applications, including detection and monitoring of bladder, kidney, or urinary-tract cancers. It also isolates bacterial DNA from both human and animal urine to study bacterial levels and types present. The kit effectively isolates DNA from both Gram-negative and Gram-positive bacteria, including E. coli, Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp., Clostridial spp., Leptospirosis spp., Chlamydia trachomatis, and Neisseria gonorrhoeae.

Typical yields vary depending on cell or bacterial density, which is influenced by health, diet, and sex. The kit is sensitive enough to isolate DNA from <10,000 CFU/mL bacteria found in healthy individuals. Purified DNA is fully digestible with all tested restriction enzymes and compatible with PCR, qPCR, and Southern blot analyses.

Kit Specifications

Storage Conditions and Stability

All solutions should be tightly sealed and stored at room temperature. The kit is stable for 2 years from shipment. Proteinase K is provided in a buffered storage solution and remains stable for up to 2 years at room temperature.

Kit Components

Supporting Data

Figure 1. Isolation and Detection of Human DNA from 1 mL Urine

Genomic DNA was isolated from exfoliated cells found in a 1 mL urine sample using Norgen’s kit.
PCR amplification using human beta-actin primers confirmed successful detection.

그래프 요약 (OCR 데이터):

이 그래프는 서로 다른 세포 농도에 따른 PCR 증폭 신호 변화를 보여줍니다.

Figure 2. Isolation and Detection of Bacterial DNA from 1 mL Urine

Bacterial genomic DNA was isolated from a 1 mL urine sample from a healthy individual.
PCR amplification confirmed detection even at low bacterial concentrations (<10,000 CFU/mL).

그래프 요약 (OCR 데이터):

이 그래프는 서로 다른 세포 농도에서의 PCR 증폭 결과를 비교한 것입니다.

Figure 3. Isolation of Genomic DNA from Gram-Positive and Gram-Negative Bacteria

Genomic DNA was isolated from urine samples spiked with B. cereus (Gram-positive) and E. coli (Gram-negative).
DNA was successfully recovered from both bacterial types.

샘플 구조 (OCR 데이터):

Documentation

• Protocol (50 prep)
• Safety Data Sheet (SDS)
• Flyer
• Urine Biomarker Research Brochure

FAQs

Q1. Why is my genomic DNA yield low?

• Few cells or bacteria may be present in urine (<10,000 CFU/mL typical for healthy individuals).
• Incomplete lysis: extend Proteinase K digestion or use fresh samples.
• Incomplete elution: ensure centrifugation at 14,000 RPM after initial spin.

Q2. Why is my genomic DNA sheared?

• Handle samples gently; minimize vortexing (≤15 sec).
• Use fresh urine to prevent degradation by DNases and proteases.

Q3. Why does DNA perform poorly in PCR?

• Inhibitors may be present in urine. Perform an additional wash with Resuspension Solution A + Proteinase K before lysis.

Q4. Can Proteinase K be purchased separately?

• Yes, available as Product No. 28229 (5 × 1 mL tubes).

Citations

Related Products

• Urine DNA Isolation Kit (Slurry Format)
• ProteoSpin™ Urine Protein Concentration Midi Kit

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