Thermo Fisher Scientific Thioredoxin 1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Immunogen sequence:
MVKQIESKTA FQEALDAAGD KLVVVDFSA

Target Information

Thioredoxins (Trx) are small, multifunctional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and several transcription factors such as p53, NF-kB, and AP-1. Trx is also capable of removing H₂O₂, particularly when coupled with methionine sulfoxide reductase or various peroxiredoxin isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.