Thermo Fisher Scientific HYPE Polyclonal Antibody, Biotin
HYPE 단백질을 인식하는 Biotin 결합 토끼 다클론 항체로, Western blot, ELISA, Immunoprecipitation에 적합합니다. 사람, 마우스, 랫트 반응성이 있으며 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:50,000 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids within region 400–450 on mouse Protein adenylyltransferase FICD |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Proprietary buffer with 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context.
The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place.
Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP.
In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at Thr-518, thereby inactivating it.
In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at Thr-518, restoring HSPA5/BiP activity.
Although it can AMPylate RhoA, Rac, and Cdc42 Rho GTPases in vitro, these are not physiological substrates.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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