Thermo Fisher Scientific AFF1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human AFF1 protein. Validated for ICC/IF applications. Recombinant human AFF1 immunogen with high specificity. Supplied in liquid form, 0.25 mg/mL, stored at 4°C or -20°C. For research use only.
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Applications and Tested Dilution
- Application: Immunocytochemistry (ICC/IF)
- Tested Dilution: 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human AFF1. Recombinant protein control fragment (Product #RP-107722) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2662943 |
Product Specific Information
Immunogen sequence:
VDLIKFIMSLK SFSDATAPTQ EKIFAVLCMR CQSILNMAMF RCKKDIAIKY SRTLNKHFES SSK
Highest antigen sequence identity to the following orthologs:
- Mouse: 73%
- Rat: 80%
Target Information
Proto-oncogene AF-4 (or FEL) is a product of a chromosomal aberration of the human gene AFF1, associated with acute leukemias. The fusion of AF-4 on chromosome band 4q21 with the mixed lineage leukemia (MLL or HRX) gene on 11q23 results in a MLL-AF-4 chimeric transcription factor. AF-4 contributes transcriptional effector properties and requires cell-specific accessory factors. MLL is involved in several chromosomal translocations associated with acute myeloid and lymphoid leukemia. The MLL-AF-4 fusion protein is expressed in all normal hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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