Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), eFluor 450, eBioscience

Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), eFluor 450, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 1 µg/test
• View 9 publications

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant Violet 480
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation available

Product Specific Information

The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated α/β-TCR-bearing T cells, a subset of γ/δ-TCR-bearing T cells, and NK cells.
CD223 is a 70 kDa type I transmembrane protein similar to CD4. A soluble form of human CD223 has been detected in serum, and mouse CD223 is proteolytically cleaved, producing a 54 kDa extracellular fragment and a 16 kDa intracellular fragment. The 54 kDa fragment can remain membrane-associated or be released as soluble CD223.

CD223 binds to MHC class II with higher affinity than CD4 and is involved in negative regulation of T-cell activation and homeostatic proliferation. It is also expressed by CD4+CD25+ regulatory T cells, suggesting a role in their regulatory function.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of stimulated mouse splenocytes at ≤1 µg/test (100 µL final volume). Optimal titration recommended for each assay.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®. Emission at 445 nm, excitation at 405 nm (Violet laser). Ensure instrument compatibility.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LAG-3 (CD223) is a 70-kDa surface glycoprotein of the Ig superfamily, homologous to CD4. It binds MHC class II with higher affinity than CD4 and negatively regulates T-cell activation and proliferation. Expressed on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells typically express higher levels than CD4+ T cells. Coexpression with CD49b identifies Type 1 regulatory T cells (Tr1) in humans and mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지