Thermo Fisher Scientific CAD Monoclonal Antibody (OTI10A3), TrueMAB
CAD 단백질을 인식하는 Mouse monoclonal antibody(OTI10A3)로 Western blot에 최적화. Human, Mouse, Rat 반응성. Lyophilized 형태로 제공되며 PBS buffer에 8% trehalose 포함. 연구용으로 de novo pyrimidine 합성 관련 단백질 분석에 적합.
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- CF812683
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Applications
- Western Blot (WB): Tested dilution 1:250–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10A3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1918–2225 of human CAD produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The de novo synthesis of pyrimidine nucleotides is essential for mammalian cell proliferation.
This gene encodes a trifunctional protein associated with the enzymatic activities of the first three enzymes in the six-step pathway of pyrimidine biosynthesis:
- Carbamoylphosphate synthetase (CPS II)
- Aspartate transcarbamoylase
- Dihydroorotase
This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, linking MAPK activation to de novo pyrimidine biosynthesis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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