Himedia Acetamide Agar, Modified (Twin Pack)

Acetamide Agar, Modified (Twin Pack) is recommended for confirmation of Pseudomonas aeruginosa in water samples.This medium is based on the unique ability for confirmation of Pseudomonas aeruginosa in water samples by the process of deamination (acrylamidase activity). This unique ability is useful in identification of various non-fermenting gram negative organisms including Pseudomonas aeruginosa, Pseudomonas aciovorans Group III (Achromobacter xylosoxidans) and Alcaligenes odorans. Acetamide deamination leads to the liberation of ammonia, which thereby increases the pH of the medium, leading to a subsequent colour change of the phenol red indicator from yellow orange to purplish red. Some strains require up to seven days to exhibit a positive reaction as they deaminate acrylamide slowly.