Thermo Fisher Scientific SIRT1 Polyclonal Antibody
SIRT1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 Immunoprecipitation에 적합합니다. Mouse와 Human 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하며, 방부제는 포함되어 있지 않습니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 5 publications |
| Immunoprecipitation (IP) | 1:50 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the carboxy terminus of mouse SirT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 316 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979977 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other sirtuin proteins.
Target Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy.
SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in histone and DNA methylation, leading to transcriptional repression.
It is essential in skeletal muscle cell differentiation and, in response to low nutrients, mediates the inhibitory effect on skeletal myoblast differentiation, which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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