Thermo Fisher Scientific beta Tubulin Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000
• View 1 publication

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:1,000
• View publication

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3
• Predicted reactivity: Mouse (92%), Cat (81%), Pig (90%), Chicken (86%), Bovine (93%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules.
Beta-III tubulin is primarily expressed in neurons and may play roles in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene cause congenital fibrosis of the extraocular muscles type 3.
Beta-III tubulin is also detected in Sertoli cells and transiently in non-neuronal embryonic tissues.
Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, affecting incorporation into microtubules.
Microtubules, composed of alpha and beta tubulin dimers (~55 kDa each), are essential for mitosis, intracellular transport, ciliary movement, and cytoskeletal structure.
Beta-tubulin is widely used as a loading control in protein detection assays such as Western blotting.

⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.