Thermo Fisher Scientific Zap-70 Polyclonal Antibody
ZAP-70 단백질을 인식하는 Thermo Fisher Scientific의 다클론 항체로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. Human과 Rat 반응성이 있으며, 항원 친화 크로마토그래피로 정제된 고순도 제품입니다. 연구용으로 T세포 수용체 신호 연구에 활용됩니다.
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Thermo Fisher Scientific Zap-70 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 269–619 of Human ZAP70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.21 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546173 |
Product Specific Information
- Recommended positive control: Jurkat cells
- Predicted reactivity: Mouse (95%), Rat (93%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T cells and natural killer cells and is essential for T-cell receptor activation. Upon TCR engagement, ZAP-70 is phosphorylated on tyrosines 292, 315, and 319 within the interdomain B region.
Phosphorylation of tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP-70 function in lymphocyte signaling, while phosphorylation at tyrosine 292 attenuates activation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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