Thermo Fisher Scientific Phospho-JAK2 (Tyr221) Polyclonal Antibody
Phospho-JAK2 (Tyr221) 폴리클로날 항체로 JAK2 인산화 상태 검출에 적합. WB, IHC, ICC, Flow, ELISA 등 다양한 응용 가능. Rabbit IgG 기반, Protein A 정제, 고순도 액상 포맷. Human, Mouse, Rat, Zebrafish 반응성.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human JNK2 around the phosphorylation site of Tyr221 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Janus kinase 2 (JAK2) is a protein tyrosine kinase involved in cytokine receptor signaling pathways and is mutated in polycythemia vera. It contains the catalytic domain (JH1) of JAK2. Activation of JAKs leads to phosphorylation of STAT proteins, which dimerize and translocate to the nucleus to regulate transcription of interferon-stimulated genes. JAK2 is required for IFN gamma-receptor complex initiation, with JAK1 acting as an amplifier. Mutations in JAK2 are associated with Budd-Chiari syndrome, Polycythemia vera, Thrombocythemia 3, myelofibrosis, and Acute myelogenous leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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