Thermo Fisher Scientific Expi293F Inducible GnTI- Cells

Gibco™ Expi293F™ Inducible GnTI- Cells

The Expi293F Inducible GnTI- cell line is derived from engineered Expi293F cells that lack N-acetylglucosaminyltransferase I (GnTI) activity, resulting in the absence of complex N-glycans. These cells stably express the tetracycline repressor (TetR) protein, enabling tetracycline-regulated protein expression.

This cell line was established by selective knockout of the GnTI gene and stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. It is a powerful tool for tetracycline-inducible, high-yield expression of homogeneously glycosylated recombinant proteins.

Features

• Suspension, high-density culture in Expi293 Expression Medium
• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells (up to 1 g/L)

For inducible expression of your protein of interest, the pcDNA5/TO mammalian expression vector or an equivalent inducible vector is required for tetracycline-regulated gene expression.

Frozen cells are supplied in a vial containing 1 mL of cells at 1 × 10⁷ viable cells/mL in Expi293 Expression Medium and 10% DMSO.
Cells should be thawed directly into Expi293 Expression Medium.

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