Thermo Fisher Scientific CD254 (RANK Ligand) Monoclonal Antibody (MIH24), Biotin, eBioscience

Thermo Fisher Scientific CD254 (RANK Ligand) Monoclonal Antibody (MIH24), Biotin, eBioscience

Applications and Tested Dilutions

Product Specifications

Product Specific Information

The MIH24 monoclonal antibody reacts with human TRANCE (TNF-related activation-induced cytokine receptor), also known as RANKL (receptor activator of NF-kappaB ligand) and OPGL (Osteoprotegerin Ligand).
TRANCE is a type II membrane protein (~35 kDa) closely related to TRAIL, FasL, and TNF. Its mRNA is upregulated by TCR stimulation, and TRANCE-deficient mice lack osteoclasts.
TRANCE plays a critical role in osteoclast differentiation and in T cell–dendritic cell and T cell–B cell interactions. The TRANCE-RANK interaction regulates expression of the anti-apoptotic molecule Bcl-xL.

Applications Reported: Flow cytometry and ELISA
Applications Tested: ELISA (titrate for optimal performance)

Target Information

This gene encodes a member of the TNF cytokine family, a ligand for osteoprotegerin, and a key factor in osteoclast differentiation and activation.
It functions as a dendritic cell survival factor and regulates T cell-dependent immune responses.
Activation of T cells induces expression of this gene, promoting osteoclastogenesis and bone resorption.
The protein activates antiapoptotic kinase AKT/PKB through a complex involving SRC and TRAF6, suggesting a role in apoptosis regulation.
Gene disruption in mice leads to severe osteopetrosis, lack of osteoclasts, defects in early T/B lymphocyte differentiation, and impaired mammary gland development during pregnancy.
Two alternatively spliced transcript variants have been identified.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.