Thermo Fisher Scientific Phospho-P53 (Thr81) Polyclonal Antibody
Phospho-P53 (Thr81) 폴리클로날 항체로 인간 및 마우스 시료에 반응. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. Protein A로 정제된 고순도 항체로 안정적 액상 형태 제공. 세포 스트레스 반응 및 p53 신호전달 연구에 적합.
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Thermo Fisher Scientific Phospho-P53 (Thr81) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Thr81 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or pyrimidine nucleotide starvation. p53 upregulation leads to transcription of genes such as p21waf1, contributing to G1 or G2/M arrest. Structurally, p53 includes an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain.
Phosphorylation at Ser15 is crucial for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 also relates to aging, oxidative stress, and cognitive functions such as learning and memory. Normally expressed at low levels, p53 is highly expressed in transformed cells, contributing to malignancy. Mutations in TP53 disrupt DNA binding and tumor suppressor activity, occurring as somatic or germline mutations (e.g., Li-Fraumeni syndrome).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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