Thermo Fisher Scientific LIG4 Monoclonal Antibody (1A4)

Applications

Tested Dilution

Publications

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1A4

Immunogen

LIG4 (AAH37491, 802 a.a. approximately 911 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DNA Ligase IV,uniProtId:P49917-1,ncbiNodeId:9606,antigenRange:802-911,antigenLength:911,antigenImageFileName:H00003981-M01A_DNA_Ligase_IV_P49917-1_House_mouse.svg,antigenImageFileNamePDP:H00003981-M01A_DNA_Ligase_IV_P49917-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Storage buffer

ascites

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: RYSWDCSPLS MFRRHTVYLD SYAVINDLST KNEGTRLAIK ALELRFHGAK VVSCLAEGVS HVIIGEDHSR VADFKAFRRT FKRKFKILKE SWVTDSIDKC ELQEENQYLI

Target Information

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.