Norgen Stool Nucleic Acid Collection and Preservation Tubes

Norgen Stool Nucleic Acid Collection and Preservation Tubes

SKU: 45660
A rapid and simple method for the collection, preservation, and room temperature shipping of stool samples.

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Features and Benefits

• Sample Homogeneity: Eliminates sample variability.
• Inactivating: Renders the sample non-infectious by preventing bacterial/fungal growth and inactivating viruses; eliminates odor.
• Ambient Temperature Preservation: Preservation for over 2 years for DNA and 7 days for RNA at room temperature.
• Cost-Effective: No cold shipping or storage required.
• Robust Preservation: Effective over a range of temperatures.
• Freezer Compatible: Tubes can be stored at -80°C to extend nucleic acid stabilization.

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Applications

• DNA and RNA Isolation: Compatible with multiple isolation methods, including Norgen’s Stool DNA, RNA, and total nucleic acid isolation kits.
• Microbiome Profiling: Reliable and reproducible source of sample for microbial detection or metagenomics analysis via NGS, qPCR, ddPCR, and other applications.
• Metabolomics: Preserved stool samples are compatible with SCFA and metabolomic analysis according to literature.

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NORGEN BIO TEK CORP.
WWW.NORGENBIOTEK.COM
MADE IN CANADA

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Extracting Biological Insights from Stool

Tips and tricks for isolating high yield and quality DNA, RNA, miRNA, and EVs from fecal samples.
NORGEN BIOTEK
ISO • ISO • ISO • GMP

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Supporting Data

Figure 1. Norgen’s Best-In-Class Preservation
Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Norgen shows the greatest similarity to frozen stool samples.

Figure 2. Integrity of Total Stool DNA and RNA Isolated from Preserved Stool Samples
DNA and RNA were isolated from preserved stool stored at room temperature (DNA at 27 months, RNA at 7 days).
27 months – Stool DNA (RT)
| M | A | B | C | D | M |
1.2% 1× TAE agarose gel
7 days – Stool RNA (RT)
| A | B | C | D |
1.2% 1× MOPS agarose gel

Figure 3. Monitoring the DNA Stability of Adenovirus Spiked into Preserved Stool Samples
Stool preservative stabilizes Adenovirus DNA up to 21 weeks at ambient temperature.

Figure 4. Hierarchical Clustering of Stool Samples at Phylum Level
Microbial composition preserved similarly to fresh stool over 2 days.

Figure 5. Difference in Top 5 Abundant Microorganisms at Phylum Level
Preserved stool maintains microbial profile closer to fresh stool than non-preserved samples.

Figure 6. Relative Abundance of Bacterial Genera
Preserved samples show close similarity to fresh stool at Day 0.

Figure 7. Correlation Between Duplicate Bacterial Metagenomic Reads
Higher consistency between fresh and preserved stool samples.

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Specifications

* RNA stability may vary depending on sample type.

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FAQs

• Preservative Volume: 4 mL (~4.56 g) per tube; scoop attached to cap.
• Tube Dimensions: 76 mm (H) × 20 mm (D).
• Material: Cap – HDPE, Stem – PS, Tube – PP.
• Compatible Kits: Stool DNA Isolation Kit, Stool Total RNA Purification Kit, Stool Nucleic Acid Isolation Kit.
• Metabolomics Compatibility: Suitable for SCFA and other metabolite analyses.
• Infant Stool Compatibility: Validated for infant feces (birth to 6 months).
• microRNA Preservation: Yes, after proper homogenization.
• Freezing: Store between -20°C and -80°C for extended RNA stability.

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Documentation

• Protocol – Stool Nucleic Acid Collection and Preservation Tubes (50 devices)
• Application Notes
• Safety Data Sheet (SDS)

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Related Products

• Stool DNA Isolation Kit
• Stool Nucleic Acid Isolation Kit
• Stool Total RNA Purification Kit
• Fecal DNA Collection & Preservation Mini Tubes

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