Thermo Fisher Scientific Ep-CAM/CD326 Monoclonal Antibody (EGP40, 837)
Ep-CAM/CD326 단일클론 항체로 인간 시료에 특이적 반응. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. 단백질 A/G 정제 및 PBS 버퍼에 보존. Ep-CAM 발현 종양 연구 및 세포 접착 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EGP40, 837 |
| Immunogen | Recombinant human TACSTD1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with Mouse or Rat.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial tissues with a molecular weight of approximately 40 kDa. It functions as a homotypic, calcium-independent cell adhesion molecule, influencing cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cell cycle-regulating genes cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is linked to increased epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for tumors of epithelial origin. It is located on the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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