Thermo Fisher Scientific Protein Kinase C alpha/PRKCA Monoclonal Antibody (133)
PKC alpha 단백질을 인식하는 단클론 항체로, Western blot 및 면역조직화학(IHC) 분석에 적합합니다. 인간, 마우스, 랫트에서 반응하며, 단백질 A/G로 정제된 액상 형태입니다. 보존제가 없으며 -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 133 |
| Immunogen | Full-length protein corresponding to PKC alpha. Recognizes sequence PQFVHPILQSAV at the C terminus at PKC alpha. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in structure, tissue distribution, localization, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family, which are Ca²⁺- and phospholipid-dependent. PKC family members phosphorylate various protein targets and play roles in multiple signaling pathways. They also act as receptors for phorbol esters, known tumor promoters. PKC alpha is involved in cellular processes such as adhesion, transformation, cell cycle control, and cardiac contractility regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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