Thermo Fisher Scientific CH25H Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 2 publications 2 publications

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

synthetic peptide directed towards the C-terminal region of human CH25H if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CH25H,uniProtId:O95992-1,ncbiNodeId:9606,antigenRange:272,antigenLength:272,antigenImageFileName:PA5-70691_CH25H_O95992-1_Rabbit.svg,antigenImageFileNamePDP:PA5-70691_CH25H_O95992-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2689560

Product Specific Information

This target displays homology in the following species: Cow: 93%; Goat: 83%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%; Yeast: 75%; Zebrafish: 86%

Target Information

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.