Thermo Fisher Scientific MCM10 Polyclonal Antibody
Human MCM10 단백질에 특이적인 Rabbit Polyclonal 항체로, WB 및 IHC(P) 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보존. 세포 주기 의존적 복제 단백질 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MCM10. Recombinant protein control fragment (Product #RP-97005). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2643811 |
Product Specific Information
Immunogen sequence:
QTTLSNLVVK GTNLIIQETR QKLGIPQKSL SCSEEFKELM DLPTCGARNL KQHLAKATAS GIMGSPKPAI KSISASALLK QQKQRMLEMR RRKSEEIQK
Highest antigen sequence identity to the following orthologs:
- Mouse: 80%
- Rat: 79%
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in replication fork formation and recruitment of other DNA replication-related proteins.
This protein interacts with MCM2, MCM6, and the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner.
Studies in Xenopus suggest that chromatin binding of this protein occurs after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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