Thermo Fisher Scientific EEA1 Polyclonal Antibody
EEA1 단백질을 인식하는 Thermo Fisher Scientific의 다클론 항체로, Western blot, IHC, ELISA 등에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 친화 크로마토그래피 정제, PBS/glycerol 버퍼 저장. 세포 내 초기 엔도솜 연구에 적합.
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Thermo Fisher Scientific EEA1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1182–1411 of human EEA1 (NP_0035572) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.13 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804168 |
Product Specific Information
Immunogen sequence:
LKAAVEQEKR NQQILKDQVK KEEEELKKEF IEKEAKLHSE IKEKEVGMKK HEENEAKLTM QITALNENLG TVKKEWQSSQ RRVSELEKQT DDLRGEIAVL EATVQNNQDE RRALLERCLK GEGEIEKLQT KVLELQRKLD NTTAAVQELG RENQSLQIKH TQALNRKWAE DNEVQNCMAC GKGFSVTVRR HHCRQCGNIF CAECSAKNAL TPSSKKPVRV CDACFNDLQG
Positive Samples:
U-87MG, HT-1080, LO2, Mouse kidney, Mouse lung, Rat brain
Cellular Location:
Cytoplasm, Early endosome membrane, Peripheral membrane protein
Target Information
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane-bound protein specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments that fuse with endocytic vesicles for redistribution of extracellular compounds. Zinc-finger-like domains are present at both amino and carboxyl termini, with the carboxyl-terminal domain conserved across several non-nuclear proteins involved in intracellular trafficking. This domain is an authentic zinc-binding domain critical for EEA1 localization. Membrane association of EEA1 depends on phosphatidyl 3-kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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