Thermo Fisher Scientific EEA1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1182-1411 of human EEA1 (NP_0035572) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEA1,uniProtId:Q15075-1,ncbiNodeId:9606,antigenRange:1182-1411,antigenLength:1411,antigenImageFileName:PA5-87460_EEA1_Q15075-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87460_EEA1_Q15075-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.13 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804168

Product Specific Information

Immunogen sequence: LKAAVEQEKR NQQILKDQVK KEEEELKKEF IEKEAKLHSE IKEKEVGMKK HEENEAKLTM QITALNENLG TVKKEWQSSQ RRVSELEKQT DDLRGEIAVL EATVQNNQDE RRALLERCLK GEGEIEKLQT KVLELQRKLD NTTAAVQELG RENQSLQIKH TQALNRKWAE DNEVQNCMAC GKGFSVTVRR HHCRQCGNIF CAECSAKNAL TPSSKKPVRV CDACFNDLQG; Positive Samples: U-87MG, HT-1080, LO2, Mouse kidney, Mouse lung, Rat brain; Cellular Location: Cytoplasm, Early endosome membrane, Peripheral membrane protein

Target Information

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.