Thermo Fisher Scientific TNP2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human TNP2(Accession Q05952), corresponding to amino acid residues Q39-H89. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TNP2,uniProtId:Q05952-1,ncbiNodeId:9606,antigenRange:39-89,antigenLength:138,antigenImageFileName:PA5-103981_TNP2_Q05952-1_Rabbit.svg,antigenImageFileNamePDP:PA5-103981_TNP2_Q05952-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2815994

Product Specific Information

Antibody detects endogenous levels of total TNP2.

Target Information

During mammalian spermiogenesis, histones are transiently replaced by several low molecular weight proteins called transition proteins (TNPs) . Transition proteins facilitate chromatin transformation from the nucleosome structure to the nucleoprotamine structure during spermatid differentiation . Transition protein-2, also known as TNP2, and TP2, maps to human chromosome 16p13.13 and encodes a highly basic nuclear protein. TNP2 is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines . TNP2 is not a critical factor for shaping of the sperm nucleus, histone displacement, initiation of chromatin condensation, binding of protamines to DNA, or fertility . However, TNP2 is necessary for maintaining the normal processing of protamine 2 and, consequently, the completion of chromatin condensation. If TNP1 is missing, TNP2 may partially compensate for TNP1, but this dysregulation of nucleoprotein replacement results in an abnormal pattern of chromatin condensation and in reduced fertility .

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.