Thermo Fisher Scientific MLH1 Monoclonal Antibody (G168-15)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

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View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:50

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

G168-15

Immunogen

Recombinant, full length protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MLH1,uniProtId:P40692-1,ncbiNodeId:9606,antigenRange:1-756,antigenLength:756,antigenImageFileName:MA1-25669_MLH1_P40692-1_House_mouse.svg,antigenImageFileNamePDP:MA1-25669_MLH1_P40692-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2145602

Product Specific Information

Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested positive controls for this product are tonsil and colon carcinoma.

Target Information

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.