Thermo Fisher Scientific OCT1 Polyclonal Antibody
OCT1 (POU2F1) 인식 토끼 폴리클로날 항체로, WB, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태. 인체 및 생쥐 반응성 확인, FFPE 조직에 citrate buffer 전처리 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 250 and 300 of human octamer-binding transcription factor 1 (OCT1, POU2F1, UniProt ID: P14859-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Rat, X. laevis, X. tropicalis, Chicken, and Pig.
Target Information
OCT1 (POU2F1) is a ubiquitously expressed transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates promoters of small nuclear RNAs (snRNA) and genes such as histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR, and PGR.
During human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator VP16 and HCFC1, enabling transcription of viral immediate early genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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