Thermo Fisher Scientific NF-YA Polyclonal Antibody
Rabbit polyclonal antibody targeting human NF-YA, validated for WB, IHC, IHC-P, and IP. Recommended dilution 1:2,000–1:10,000 (WB). Supplied as liquid, 0.20 mg/mL, stored at 4°C. Predicted reactivity with rat and bovine. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 333 and 347 of human nuclear transcription factor Y, alpha (NFYA; UniProt ID: P23511-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat and Bovine
Target Information
The SMN1 gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements, making it prone to rearrangements and deletions. The telomeric and centromeric copies of this gene encode the same protein — survival motor neuron protein. The SMN complex plays a catalytic role in assembling small nuclear ribonucleoproteins, the building blocks of the spliceosome. Mutations in the SMN1 gene are known to cause spinal muscular atrophy types 1 and 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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