Thermo Fisher Scientific SKA3 Polyclonal Antibody
SKA3 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunoprecipitation에 적합합니다. 인간 SKA3 단백질의 362-412번 잔기 영역을 면역원으로 사용하였으며, 액상형 1 mg/mL 농도로 제공됩니다. 4°C에서 보관하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 362 to 412 of Human Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from the date of receipt.
Target Information
Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, allowing segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for proper chromosome attachment to spindle MTs.
A spindle and kinetochore complex composed of SKA1, SKA2, and SKA3 is required for stable KT–MT interactions and timely anaphase onset. Depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged metaphase-like state. SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, indicating the SKA complex plays a key role in spindle checkpoint silencing and chromosome cohesion during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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