Thermo Fisher Scientific Phospho-c-Kit (Tyr719) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:100–1:500

ELISA

• Tested Concentration: 1 µg/mL

Product Specifications

Product Specific Information

• Positive test controls include: NIH/3T3
• Localization: Cell membrane, Cytoplasm, Single-pass type I membrane protein
• Immunogen sequence: TNEYM

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
KIT was identified as the cellular homolog of the feline sarcoma viral oncogene v-kit.
Together with its ligand, KIT regulates growth and activation of various hemopoietic and non-hemopoietic cells.

Mutations in KIT are associated with:

• Gastrointestinal stromal tumors
• Mast cell disease
• Acute myelogenous leukemia
• Piebaldism

Deregulation of KIT receptor TK by the activation loop mutation D816V is a focal point in therapeutic strategies against neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract cajal cells.
Upon SCF (stem cell factor) binding, c-kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues including Tyr703.
This enables Grb2 docking and activation of the Ras/ERK signaling pathway.
SCF/c-kit activates PI3K, PLC-γ, and JAK/STAT pathways.
Receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.