Applied Biosystems™

TaqMan™ Protein Expression Core Reagents Kit

Applied Biosystems™ TaqMan™ Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited자세히 알아보기

Applied Biosystems™ TaqMan™ Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan™ Protein Expression Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan™ protein expression assays. The Universal PCR Assay contains optimized concentrations of TaqMan™ probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems™ real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan™ Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan™ Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry. Key features of the TaqMan™ Protein Expression Assay:
• Quantitates protein expression using gold standard TaqMan™ 5` nuclease chemistry
• Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform
• Allows greater sensitivity and a more quantitative readout than western blots and immunostaining.

사양

용도(장비)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode

제품라인TaqMan™

용도(애플리케이션)Proximity Ligation Assay

검출 방법Primer-Probe Detection

라벨 또는 염료FAM

제품 유형Reagents for Protein Expression

수량100 Reactions

샘플 종류Cells

핫 스타트No

패시브 참조 염료ROX (Pre-mixed)

중합효소AmpliTaq™ Fast DNA Polymerase

프라이머 프로브 호환성TaqMan™

PCR 방법qPCR Protein Quantification

순도 또는 품질 등급UP (Ultra Pure)

반응 속도Fast

타겟 특이도Not Target-Specific

Unit SizeEach